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western blot blocking buffer  (LI-COR)


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    LI-COR western blot blocking buffer
    Western Blot Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/western+blot+blocking+buffer/TBS+BLOCKING+BUFFER+AND+PVDF+KIT/10__3390_slash_toxins18030134-250-4-8
    Average 99 stars, based on 176 article reviews
    western blot blocking buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 ◦C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 °C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).

    Blocking Assay:

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 ◦C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 °C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).

    Incubation:

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 ◦C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).

    Article Title: RTP004 Peptide Binds to Botulinum Neurotoxin, Increases Cell Surface Binding, and Enhances Cellular SNAP-25 Cleavage
    Article Snippet: Lysates (40 μg total protein/lane) were resolved on a 4% to 20% Tris-glycine gel (Thermo Fisher Scientific) and run at 100 V for 2 h at room temperature followed by transfer to 0.22 μM nitrocellulose membranes (LI-COR). .. Membranes were blocked with Western blot blocking buffer (LI-COR) for 1 h at room temperature and incubated with primary antibodies mouse anti-SNAP-25 (Synaptic Systems, Göttingen, Germany; cat# 111 111, 1:2000), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, cat# PA1-16777, 1:500 or MA5-15738, 1:500) and actin-1 (ACTA1; Thermo Fisher Scientific, PA5-78715) overnight at 4 °C. .. Membranes were washed with 1X Tris Buffered Saline with Tween (TBS-T) (Thermo Fisher Scientific) and incubated in secondary antibody goat anti-mouse IRDye 800 or goat anti-rabbit IRDye (LI-COR) for 60 min. Membranes were washed in TBS-T and scanned in Odyssey Infrared Imaging system (LI-COR).



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    Image Search Results


    A) Diagram of STEAP1 constructs. mEGFP: monomeric enhanced green fluorescent protein. B) Cell viability and VCD (Viable Cell Density) of cells expressing STEAP1 at different culturing timepoints. C) Western blot analysis of STEAP1 expression levels in relation to varying culturing durations. The asterisk denotes bands of STEAP1 positioned at the expected molecular weight. Arrows indicate bands of STEAP1 corresponding to the size of dimers and trimers. D) Measurement of STEAP1 expression levels in cells treated with heme additives (0.5 mM 5-Aminolevulinic acid HCl, 1 μM Hemin-Cl, and 5 μM Fe(III)Cl) or not via western blotting. An equal number of cells were used to prepare samples for each lane. E) FSEC analysis of the oligomeric status of STEAP1-mEGFP in cells treated with heme additives compared to those without additives. F) Cryo-EM structure of STEAP1 homotrimers (PDB: 8UCD), corresponding to the trimeric peak in . The heme moiety is shown in red, lipid in pink, and bound FAD in magenta.

    Journal: bioRxiv

    Article Title: Fine-tuning STEAP1 protein expression and purification to preserve its conformation and function

    doi: 10.64898/2026.02.16.706263

    Figure Lengend Snippet: A) Diagram of STEAP1 constructs. mEGFP: monomeric enhanced green fluorescent protein. B) Cell viability and VCD (Viable Cell Density) of cells expressing STEAP1 at different culturing timepoints. C) Western blot analysis of STEAP1 expression levels in relation to varying culturing durations. The asterisk denotes bands of STEAP1 positioned at the expected molecular weight. Arrows indicate bands of STEAP1 corresponding to the size of dimers and trimers. D) Measurement of STEAP1 expression levels in cells treated with heme additives (0.5 mM 5-Aminolevulinic acid HCl, 1 μM Hemin-Cl, and 5 μM Fe(III)Cl) or not via western blotting. An equal number of cells were used to prepare samples for each lane. E) FSEC analysis of the oligomeric status of STEAP1-mEGFP in cells treated with heme additives compared to those without additives. F) Cryo-EM structure of STEAP1 homotrimers (PDB: 8UCD), corresponding to the trimeric peak in . The heme moiety is shown in red, lipid in pink, and bound FAD in magenta.

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    Techniques: Construct, Expressing, Western Blot, Molecular Weight, Cryo-EM Sample Prep